Identification of native Dendrobium species in Thailand by PCR-RFLP of rDNA-ITS and chloroplast DNA
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چکیده
منابع مشابه
PCR - RFLP patterns for the differentiation of the Fusarium species in virtue of ITS rDNA
Background and Purpose: The Fusarium species are among the most important fungi in the medical, veterinary and agricultural fields. Materials and Methods: In the present study, 172 strains of these fungi have been analyzed. The high molecular weight DNAs were extracted from 23 reference strains as well as from 149 isolated Fusarium species. Using the designed nucleotide primers from rDNA of Fus...
متن کاملDetection and identification of Malassezia species in domestic animals and aquatic birds by PCR-RFLP
The present study aimed at detection and species-level identification of the Malassezia yeasts in domestic animals and aquatic birds by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Samples were collected using tape strips and swabs from 471 animals including 97 horses, 102 cattle, 105 sheep, 20 camels, 60 dogs, 30 cats, 1 hamster, 1 squirrel, 50 aquatic birds a...
متن کاملPCR - RFLP patterns for the differentiation of the Fusarium species in virtue of ITS rDNA
BACKGROUND AND PURPOSE The Fusarium species are among the most important fungi in the medical, veterinary and agricultural fields. MATERIALS AND METHODS In the present study, 172 strains of these fungi have been analyzed. The high molecular weight DNAs were extracted from 23 reference strains as well as from 149 isolated Fusarium species. Using the designed nucleotide primers from rDNA of Fus...
متن کاملIdentification of Mycobacteria species in cutaneous lesions of Sarcoidosis by PCR-Restriction Ragment Length Polymorphism (PCR-RFLP) method
Background: Sarcoidosis is a granulomatous multisystem disease of unknown etiology. It has recently been tired to detect Mycobacteria genome in biopsy specimens of patients with sarcoidosis by Polymorphism chain reaction method. Objective: To detect and identify Mycobacteria species in cutaneous lesions of the patients with sarcoidosis by PCR-RFLP. Patients and methods: 20 patients with clinica...
متن کاملIdentification of gadoid species (Pisces, Gadidae) by PCR-RFLP analysis.
A rapid PCR-RFLP analysis was optimized to identify the presence of 3 closely related gadoid fish species: Alaska pollack Theragra chalcogramma, Pacific cod Gadus macrocephalus and Atlantic cod Gadus morhua in commercial seafood products. Gadoid universal primers were designed for PCR amplification of a 558-bp fragment encoding the mitochondrial cytochrome b gene. Without purification of the PC...
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ژورنال
عنوان ژورنال: ScienceAsia
سال: 2014
ISSN: 1513-1874
DOI: 10.2306/scienceasia1513-1874.2014.40.113